Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood.
Identifieur interne : 000306 ( Main/Exploration ); précédent : 000305; suivant : 000307Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood.
Auteurs : Manzar Abbas [République populaire de Chine] ; Ilona Peszlen [États-Unis] ; Rui Shi [États-Unis] ; Hoon Kim [États-Unis] ; Rui Katahira [États-Unis] ; Kabindra Kafle [États-Unis] ; Zhouyang Xiang [République populaire de Chine] ; Xiong Huang [République populaire de Chine] ; Douyong Min [République populaire de Chine] ; Makarem Mohamadamin [États-Unis] ; Chenmin Yang [États-Unis] ; Xinren Dai [République populaire de Chine] ; Xiaojing Yan [République populaire de Chine] ; Sunkyu Park [États-Unis] ; Yun Li [République populaire de Chine] ; Seong H. Kim [États-Unis] ; Mark Davis [États-Unis] ; John Ralph [États-Unis] ; Ronald R. Sederoff [États-Unis] ; Vincent L. Chiang [États-Unis, République populaire de Chine] ; Quanzi Li [République populaire de Chine]Source :
- Tree physiology [ 1758-4469 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- genetics : Plants, Genetically Modified, Populus, Xylem.
- Cell Wall, Gene Expression Regulation, Plant, Wood.
Abstract
Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.
DOI: 10.1093/treephys/tpz020
PubMed: 31211386
Affiliations:
- République populaire de Chine, États-Unis
- Caroline du Nord, Guangdong, Pennsylvanie, Wisconsin
- Jiangmen, Pékin, University Park (Pennsylvanie)
- Université d'État de Pennsylvanie
Links toward previous steps (curation, corpus...)
Le document en format XML
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sortKey="Mohamadamin, Makarem" sort="Mohamadamin, Makarem" uniqKey="Mohamadamin M" first="Makarem" last="Mohamadamin">Makarem Mohamadamin</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Yang, Chenmin" sort="Yang, Chenmin" uniqKey="Yang C" first="Chenmin" last="Yang">Chenmin Yang</name><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and 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Nord</region></placeName></affiliation></author><author><name sortKey="Li, Yun" sort="Li, Yun" uniqKey="Li Y" first="Yun" last="Li">Yun Li</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Kim, Seong H" sort="Kim, Seong H" uniqKey="Kim S" first="Seong H" last="Kim">Seong H. 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Sederoff</name><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31211386</idno><idno type="pmid">31211386</idno><idno type="doi">10.1093/treephys/tpz020</idno><idno type="wicri:Area/Main/Corpus">000837</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000837</idno><idno type="wicri:Area/Main/Curation">000837</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000837</idno><idno type="wicri:Area/Main/Exploration">000837</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood.</title><author><name sortKey="Abbas, Manzar" sort="Abbas, Manzar" uniqKey="Abbas M" first="Manzar" last="Abbas">Manzar Abbas</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Peszlen, Ilona" sort="Peszlen, Ilona" uniqKey="Peszlen I" first="Ilona" last="Peszlen">Ilona Peszlen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name><affiliation wicri:level="2"><nlm:affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Katahira, Rui" sort="Katahira, Rui" uniqKey="Katahira R" first="Rui" last="Katahira">Rui Katahira</name><affiliation wicri:level="1"><nlm:affiliation>National Bioenergy Center, NREL, Golden, Co, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Bioenergy Center, NREL, Golden, Co</wicri:regionArea><wicri:noRegion>Co</wicri:noRegion></affiliation></author><author><name sortKey="Kafle, Kabindra" sort="Kafle, Kabindra" uniqKey="Kafle K" first="Kabindra" last="Kafle">Kabindra Kafle</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Xiang, Zhouyang" sort="Xiang, Zhouyang" uniqKey="Xiang Z" first="Zhouyang" last="Xiang">Zhouyang Xiang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Huang, Xiong" sort="Huang, Xiong" uniqKey="Huang X" first="Xiong" last="Huang">Xiong Huang</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Min, Douyong" sort="Min, Douyong" uniqKey="Min D" first="Douyong" last="Min">Douyong Min</name><affiliation wicri:level="1"><nlm:affiliation>Light Industry and Food Engineering College, Guangxi University, Nanning, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Light Industry and Food Engineering College, Guangxi University, Nanning</wicri:regionArea><wicri:noRegion>Nanning</wicri:noRegion></affiliation></author><author><name sortKey="Mohamadamin, Makarem" sort="Mohamadamin, Makarem" uniqKey="Mohamadamin M" first="Makarem" last="Mohamadamin">Makarem Mohamadamin</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Yang, Chenmin" sort="Yang, Chenmin" uniqKey="Yang C" first="Chenmin" last="Yang">Chenmin Yang</name><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Dai, Xinren" sort="Dai, Xinren" uniqKey="Dai X" first="Xinren" last="Dai">Xinren Dai</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yan, Xiaojing" sort="Yan, Xiaojing" uniqKey="Yan X" first="Xiaojing" last="Yan">Xiaojing Yan</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Park, Sunkyu" sort="Park, Sunkyu" uniqKey="Park S" first="Sunkyu" last="Park">Sunkyu Park</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Li, Yun" sort="Li, Yun" uniqKey="Li Y" first="Yun" last="Li">Yun Li</name><affiliation wicri:level="3"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Kim, Seong H" sort="Kim, Seong H" uniqKey="Kim S" first="Seong H" last="Kim">Seong H. Kim</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA</wicri:regionArea><placeName><region type="state">Pennsylvanie</region><settlement type="city">University Park (Pennsylvanie)</settlement></placeName><orgName type="university">Université d'État de Pennsylvanie</orgName></affiliation></author><author><name sortKey="Davis, Mark" sort="Davis, Mark" uniqKey="Davis M" first="Mark" last="Davis">Mark Davis</name><affiliation wicri:level="1"><nlm:affiliation>National Bioenergy Center, NREL, Golden, Co, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>National Bioenergy Center, NREL, Golden, Co</wicri:regionArea><wicri:noRegion>Co</wicri:noRegion></affiliation></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Sederoff, Ronald R" sort="Sederoff, Ronald R" uniqKey="Sederoff R" first="Ronald R" last="Sederoff">Ronald R. Sederoff</name><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name><affiliation wicri:level="3"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Research Institute of Forestry, Chinese Academy of Forestry, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (genetics)</term><term>Wood (MeSH)</term><term>Xylem (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bois (MeSH)</term><term>Paroi cellulaire (MeSH)</term><term>Populus (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Xylème (génétique)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Végétaux génétiquement modifiés</term><term>Xylème</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Wall</term><term>Gene Expression Regulation, Plant</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bois</term><term>Paroi cellulaire</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31211386</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>01</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Involvement of CesA4, CesA7-A/B and CesA8-A/B in secondary wall formation in Populus trichocarpa wood.</ArticleTitle><Pagination><MedlinePgn>73-89</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpz020</ELocationID><Abstract><AbstractText>Cellulose synthase A genes (CesAs) are responsible for cellulose biosynthesis in plant cell walls. In this study, functions of secondary wall cellulose synthases PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B were characterized during wood formation in Populus trichocarpa (Torr. & Gray). CesA RNAi knockdown transgenic plants exhibited stunted growth, narrow leaves, early necrosis, reduced stature, collapsed vessels, thinner fiber cell walls and extended fiber lumen diameters. In the RNAi knockdown transgenics, stems exhibited reduced mechanical strength, with reduced modulus of rupture (MOR) and modulus of elasticity (MOE). The reduced mechanical strength may be due to thinner fiber cell walls. Vessels in the xylem of the transgenics were collapsed, indicating that water transport in xylem may be affected and thus causing early necrosis in leaves. A dramatic decrease in cellulose content was observed in the RNAi knockdown transgenics. Compared with wildtype, the cellulose content was significantly decreased in the PtrCesA4, PtrCesA7 and PtrCesA8 RNAi knockdown transgenics. As a result, lignin and xylem contents were proportionally increased. The wood composition changes were confirmed by solid-state NMR, two-dimensional solution-state NMR and sum-frequency-generation vibration (SFG) analyses. Both solid-state nuclear magnetic resonance (NMR) and SFG analyses demonstrated that knockdown of PtrCesAs did not affect cellulose crystallinity index. Our results provided the evidence for the involvement of PtrCesA4, PtrCesA7-A/B and PtrCesA8-A/B in secondary cell wall formation in wood and demonstrated the pleiotropic effects of their perturbations on wood formation.</AbstractText><CopyrightInformation>© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Abbas</LastName><ForeName>Manzar</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peszlen</LastName><ForeName>Ilona</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Science, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Hoon</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Katahira</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>National Bioenergy Center, NREL, Golden, Co, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kafle</LastName><ForeName>Kabindra</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Zhouyang</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Xiong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Min</LastName><ForeName>Douyong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Light Industry and Food Engineering College, Guangxi University, Nanning, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mohamadamin</LastName><ForeName>Makarem</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Chenmin</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Xinren</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Xiaojing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Sunkyu</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yun</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Seong H</ForeName><Initials>SH</Initials><AffiliationInfo><Affiliation>Department of Chemical Engineering and Materials Research Institute, The Pennsylvania State University, University Park, PA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davis</LastName><ForeName>Mark</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>National Bioenergy Center, NREL, Golden, Co, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, University of Wisconsin, Madison, Wisconsin, WI, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sederoff</LastName><ForeName>Ronald R</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent L</ForeName><Initials>VL</Initials><AffiliationInfo><Affiliation>Department of Forest Biomaterials, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Forest Biotechnology Group, Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Quanzi</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Populus trichocarpa
</Keyword><Keyword MajorTopicYN="Y">RNAi</Keyword><Keyword MajorTopicYN="Y">cellulose</Keyword><Keyword MajorTopicYN="Y">secondary cell wall</Keyword><Keyword MajorTopicYN="Y">wood formation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>09</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>02</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31211386</ArticleId><ArticleId IdType="pii">5520187</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpz020</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Caroline du Nord</li><li>Guangdong</li><li>Pennsylvanie</li><li>Wisconsin</li></region><settlement><li>Jiangmen</li><li>Pékin</li><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Abbas, Manzar" sort="Abbas, Manzar" uniqKey="Abbas M" first="Manzar" last="Abbas">Manzar Abbas</name></noRegion><name sortKey="Abbas, Manzar" sort="Abbas, Manzar" uniqKey="Abbas M" first="Manzar" last="Abbas">Manzar Abbas</name><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Dai, Xinren" sort="Dai, Xinren" uniqKey="Dai X" first="Xinren" last="Dai">Xinren Dai</name><name sortKey="Huang, Xiong" sort="Huang, Xiong" uniqKey="Huang X" first="Xiong" last="Huang">Xiong Huang</name><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name><name sortKey="Li, Quanzi" sort="Li, Quanzi" uniqKey="Li Q" first="Quanzi" last="Li">Quanzi Li</name><name sortKey="Li, Yun" sort="Li, Yun" uniqKey="Li Y" first="Yun" last="Li">Yun Li</name><name sortKey="Min, Douyong" sort="Min, Douyong" uniqKey="Min D" first="Douyong" last="Min">Douyong Min</name><name sortKey="Xiang, Zhouyang" sort="Xiang, Zhouyang" uniqKey="Xiang Z" first="Zhouyang" last="Xiang">Zhouyang Xiang</name><name sortKey="Yan, Xiaojing" sort="Yan, Xiaojing" uniqKey="Yan X" first="Xiaojing" last="Yan">Xiaojing Yan</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Peszlen, Ilona" sort="Peszlen, Ilona" uniqKey="Peszlen I" first="Ilona" last="Peszlen">Ilona Peszlen</name></region><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Davis, Mark" sort="Davis, Mark" uniqKey="Davis M" first="Mark" last="Davis">Mark Davis</name><name sortKey="Kafle, Kabindra" sort="Kafle, Kabindra" uniqKey="Kafle K" first="Kabindra" last="Kafle">Kabindra Kafle</name><name sortKey="Katahira, Rui" sort="Katahira, Rui" uniqKey="Katahira R" first="Rui" last="Katahira">Rui Katahira</name><name sortKey="Kim, Hoon" sort="Kim, Hoon" uniqKey="Kim H" first="Hoon" last="Kim">Hoon Kim</name><name sortKey="Kim, Seong H" sort="Kim, Seong H" uniqKey="Kim S" first="Seong H" last="Kim">Seong H. Kim</name><name sortKey="Mohamadamin, Makarem" sort="Mohamadamin, Makarem" uniqKey="Mohamadamin M" first="Makarem" last="Mohamadamin">Makarem Mohamadamin</name><name sortKey="Park, Sunkyu" sort="Park, Sunkyu" uniqKey="Park S" first="Sunkyu" last="Park">Sunkyu Park</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Sederoff, Ronald R" sort="Sederoff, Ronald R" uniqKey="Sederoff R" first="Ronald R" last="Sederoff">Ronald R. Sederoff</name><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name><name sortKey="Yang, Chenmin" sort="Yang, Chenmin" uniqKey="Yang C" first="Chenmin" last="Yang">Chenmin Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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